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Filtered Search Results
Thermo Scientific™ AdeI (DraIII) (10 U/μL)
Cut at CACNNN^GTG sites with AdeI (DraIII) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: DraIII).
Thermo Scientific™ BseGI (BtsCI) (10 U/μL)
Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | BseGI (BtsCI) |
| Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
| Research Category | Scarless Cloning |
| Compatible Buffer | 10X Buffer Tango |
| Type IIS RE | Yes |
| Optimal Reaction Temperature | 55°C |
| Concentration | 10 U/μL |
Thermo Scientific™ SatI (Fnu4HI) (10 U/μL)
The SatI (Fnu4HI) restriction enzyme recognizes GC^NGC sites and cuts best at 37°C in G buffer (Isoschizomers: Fnu4HI, Fsp4HI, ItaI).
| Sensitive to Heat Inactivation | Yes |
|---|---|
| Product Type | Restriction Enzyme |
| Enzyme | SatI (Fnu4HI) |
| Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
| Research Category | Traditional Cloning |
| Compatible Buffer | 10X Buffer G |
| Type IIS RE | No |
| Optimal Reaction Temperature | 37°C |
| Concentration | 10 U/μL |
Thermo Scientific™ BseMII (BspCNI) (1 U/μL)
Cut at CTCAG(10/8)^ sites with BseMII (BspCNI) restriction enzyme, which performs best at 55°C in Tango(+SAM) buffer (Isoschizomers: BspCNI).
Thermo Scientific™ Buffer G (10X)
Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer G, premixed with BSA for enhanced stability.
Thermo Scientific™ MvaI (BstNI) (10 U/μL)
Cut at CC^WGG sites with MvaI (BstNI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AjnI, BseBI, Bst2UI, BstNI, BstOI, Psp6I, PspGI).
Thermo Scientific™ Ecl136II (EcoICRI) (10 U/μL)
Cut at GAG^CTC sites with Ecl136II (EcoICRI) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ DraI (10 U/μL)
Thermo Scientific™ DraI is available as a FastDigest™ enzyme which is used for rapid DNA digestion.
Thermo Scientific™ FastDigest Ecl136II
Cut at GAG^CTC sites with Thermo Scientific™ FastDigest™ Ecl136II, which performs best using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Thermo Scientific™ SmoI (SmlI) (10 U/μL)
Cut at C^TYRAG sites with SmoI (SmlI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: SmlI).
Thermo Scientific™ FastDigest SduI
Cut at GDGCH^C sites with Thermo Scientific™ FastDigest™ Bsp1286I (SduI), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: MhlI).
Thermo Scientific™ FastDigest EcoO109I
Cut at RG^GNCCY sites with Thermo Scientific™ FastDigest™ EcoO109I, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: DraII).
Thermo Scientific™ FastDigest Psp1406I
Cut at AA^CGTT sites with Thermo Scientific™ FastDigest™ AclI (Psp1406I), which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest Eam1104I
Cut at CTCTTC(1/4)^ sites with Thermo Scientific™ FastDigest™ EarI (Eam1104I), which performs best using universal FastDigest Buffer (Isoschizomers: Bst6I).
Thermo Scientific™ FastDigest MnlI (IIs class)
The FastDigest MnlI restriction enzyme recognizes CCTC(7/6)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.